Table 2 Primers for Real-time PCR
From: FZD5 prevents epithelial-mesenchymal transition in gastric cancer
| Genes or binding sites | Primer (5′ to 3′) |
|---|---|
| CDH1-forward | GCCATCGCTTACACCATCCTCAG |
| CDH1-reverse | CTCTCTCGGTCCAGCCCAGTG |
| ELF3-forward | ATGGTTTTCGTGACTGCAAGAA |
| ELF3-reverse | CAGTACTCTTTGCTCAGCTTTC |
| FZD5-forward | TCCTCTGCATGGATTACAACC |
| FZD5-reverse | GACACTTGCACACGAACG |
| GAPDH-forward | CAGGAGGCATTGCTGATGAT |
| GAPDH-reverse | GAAGGCTGGGGCTCATTT |
| LRP5-forward | CCCTACATCATTCGAGGAATGG |
| LRP5-reverse | CCGAGTTCAAATCCAGGTAGTA |
| VIM-forward | GGTGGACCAGCTAACCAACG |
| VIM-reverse | TTGCAGGGTGTTTTCGGCTT |
| ZEB1-forward | CAGGCAAAGTAAATATCCCTGC |
| ZEB1-reverse | GGTAAAACTGGGGAGTTAGTCA |
| SNAI2 BS1-forward | ATTTGGGCGGCTACTATGT |
| SNAI2 BS1-reverse | CCTTACAACAATTCTGAGGC |
| SNAI2 BS2-forward | ACCTGCCTCAGAATTGTTG |
| SNAI2 BS2-reverse | GAACTCCTGACACCGTGAT |
| TEAD1 BS1-forward | GTCCAGTCCCTATCCCTT |
| TEAD1 BS1-reverse | AAATGCCTCTTTTCTATGTG |
| TEAD1 BS2-forward | GGCAGGATAATCGCTTGA |
| TEAD1 BS2-reverse | GGATACTTACTGAGCCCTTTAC |